AnalyzeDirect Francesca Ruscitti et al. Quantification of Lung Fibrosis in IPF-Like Mouse Model and Pharmacological Response to Treatment by Micro-Computed Tomography. Frontiers in Pharmacology.
Publication Date
July 21, 2020
How Analyze was Used
“For each examination, a stack of 512 cross-sectional images stored in unsigned 16-bit file format was produced. The reconstructed datasets were analyzed using Perkin Elmer Analyze software (Analyze 12.0). A semi-automatic segmentation was used to define airways and total lung volumes. For quantitative assessment of the lung parenchyma, Hounsfield Unit (HU) clinical ranges (Gattinoni et al., 2001) were applied on rescaled HU images. Airways (AW) and blood vessels (BV) were segmented and used as reference points for the alignment with 2D histological sections, and lung tissue divided in poorly- ([−500, −100] HU) and normo-aerated ([−900, −500] HU).”
Keywords
Lung fibrosis, Animal model, Mice, Bleomycin, Micro-CT, Nintedanib, Idiopathic pulmonary fibrosis, Drug discovery
Author Affiliation(s)
Chiesi Farmaceutici, Parma, Italy
