AnalyzeDirect

Gao J, Du P, O’Grady G, Archer R, Farrugia G, Gibbons SJ, Cheng LK. Numerical metrics for automated quantification of interstitial cell of Cajal network structural properties. J R Soc Interface. September 2013;10(86):20130421.

Full Text – Open Access

Publication Date
September 2013

How Analyze was Used
“Confocal image slices were sequentially taken throughout the transmural depth of the muscularis propria in fine steps of 0.2–0.3 μm and were volume-rendered in three dimensions. Bitmaps of the positively labelled structures were then obtained using the Analyze software (AnalyzeDirect, Overland Park, KS).”

Keywords
Animals
Chloride Channels/metabolism
Confocal Microscopy
Image Processing, Computer-Assisted/methods
Interstitial Cells of Cajal/cytology/metabolism
Maturation
Mice
Mice, Knockout
Models, Biological
Post-Natal
Pruning
Receptors, Serotonin, 5-HT2/metabolism
Remodelling

Author Affiliation(s)
Auckland Bioengineering Institute, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand. (JG, PD, GO, LCK)

Department of Surgery, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand. (GO)

Department of Engineering Science, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand. (RA)

Enteric Neuroscience Program, Division of Gastroenterology and Hepatology, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, US. (GF, SJG)

Department of Surgery, Vanderbilt University, Nashville, TN 37240, US. (LCK)

ID# 400

Tags: Animals, Biological Models, Chloride Channels/metabolism, Computer-Assisted Image Processing/methods, Confocal Microscopy, Interstitial Cells of Cajal/cytology/metabolism, Knockout Mice, Maturation, Mice, Post-Natal, Pruning, Remodelling, Serotonin 5-HT2 Receptors/metabolism