AnalyzeDirect Pirko I, Chen Y, Lohrey AK, McDole J, Gamez JD, Allen KS, Pavelko KD, Lindquist DM, Dunn RS, Macura SI, Johnson AJ. Contrasting roles for CD4 vs. CD8 T-cells in a murine model of virally induced “T1 black hole” formation. PLoS One. February 2012;7(2):e31459.
Full Text – Open Access
Publication Date
February 2012
How Analyze was Used
“In vivo 3-dimensional MRI images were acquired using a 7 Tesla small animal MRI system. For image analysis, we used semi-automated methods in Analyze 9.1; transfer efficiency was monitored using FACS of brain infiltrating lymphocytes.”
Keywords
Adoptive Transfer
Animals
Axons/pathology
CD4-Positive T-Lymphocytes/pathology/virology
CD8-Positive T-Lymphocytes/pathology/virology
Central Nervous System/pathology
Inflammation
Magnetic Resonance Imaging (MRI)
Mice
Models, Animal
Multiple Sclerosis/pathology
Theilovirus
Author Affiliation(s)
Department of Neurology, Mayo Clinic, Rochester, MN, US. (IP, JDG)
Department of Neurology, University of Cincinnati, Cincinnati, OH, US. (YC, AKL)
Department of Pathology and Immunology, Washington University in St. Louis, St. Louis, MO, US. (JM)
Department of Immunology, Mayo Clinic, Rochester, MN, US. (KSA, KDP, AJJ)
Imaging Research Center, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH, US. (DML, RSD)
Department of Biochemistry, NMR Core Facility, Mayo Clinic, Rochester, MN, US. (SIM)
ID# 604
Tags: Adoptive Transfer, Animal Models, Animals, Axons/pathology, CD4-Positive T-Lymphocytes/pathology/virology, CD8-Positive T-Lymphocytes/pathology/virology, Central Nervous System/pathology, Inflammation, Magnetic Resonance Imaging (MRI), Mice, Multiple Sclerosis/pathology, Theilovirus