AnalyzeDirect Gong Y, Bourhis E, Chiu C, Stawicki S, DeAlmeida VI, Liu BY, Phamluong K, Cao TC, Carano RA, Ernst JA, Solloway M, Rubinfeld B, Hannoush RN, Wu Y, Polakis P, Costa M. Wnt isoform-specific interactions with coreceptor specify inhibition or potentiation of signaling by LRP6 antibodies. PLoS One. September 2010;5(9):e12682.
Full Text – Open Access
Publication Date
September 2010
How Analyze was Used
“Micro-CT scans were analyzed with Analyze (AnalyzeDirect Inc., Lenexa, KS, USA). Maximum-intensity projections and three-dimensional surface renderings in the transverse plane were created for each sample. Parietal bone borders were manually defined within micro-CT images in order to segment the parietal region. Within this region, sample volume and mean bone mineral density (BMD) were calculated. A threshold of 0.3 gm-HA/cm3 was applied to the region in order to calculate the mean BMD of only calcified tissue within the region. The threshold was also used to calculate percentage calcified volume of the parietal region by dividing the number of calcified voxels over the total voxels for the parietal region. The following parameters were analyzed for each sample: parietal region volume, BMD of calcified voxels of the parietal region, and parietal region percentage calcified.”
Keywords
Animals
Antibodies/pharmacology
Cell Line
Down-Regulation/drug effects
Humans
LDL-Receptor Related Proteins/genetics/immunology/metabolism
Low Density Lipoprotein Receptor-Related Protein-6
Mice
Protein Binding/drug effects
Protein Isoforms/genetics/metabolism
Receptors, G-Protein-Coupled/genetics/metabolism
Signal Transduction/drug effects
Species Specificity
Up-Regulation/drug effects
Wnt Proteins/genetics/metabolism
Author Affiliation(s)
Department of Cancer Targets, Genentech Research & Early Development, South San Francisco, California, US. (YG, VID, BYL, BR, PP, MC)
Department of Protein Engineering, Genentech Research & Early Development, South San Francisco, California, US. (EB, JAE, RNH)
Department of Antibody Engineering, Genentech Research & Early Development, South San Francisco, California, US. (CC, SS, YW)
Department of Molecular Biology, Genentech Research & Early Development, South San Francisco, California, US. (KP, MS)
Department of Tumor Biology and Angiogenesis, Genentech Research & Early Development, South San Francisco, California, US. (TCC, RADC)
Department of Protein Chemistry, Genentech Research & Early Development, South San Francisco, California, US. (JAE)
ID# 1156
Tags: Animals, Antibodies/pharmacology, Cell Line, Down-Regulation/drug effects, G-Protein-Coupled Receptors/genetics/metabolism, Humans, LDL-Receptor Related Proteins/genetics/immunology/metabolism, Low Density Lipoprotein Receptor-Related Protein-6, Mice, Protein Binding/drug effects, Protein Isoforms/genetics/metabolism, Signal Transduction/drug effects, Species Specificity, Up-Regulation/drug effects, Wnt Proteins/genetics/metabolism