AnalyzeDirect Liu Y, Uberti MG, Dou H, Banerjee R, Grotepas CB, Stone DK, Rabinow BE, Gendelman HE, Boska MD. Ingress of blood-borne macrophages across the blood-brain barrier in murine HIV-1 encephalitis. J Neuroimmunol. August 2008;200(1-2):41-52.
Full Text – Open Access
Publication Date
August 2008
How Analyze was Used
“Individual blockface slices were aligned to reconstruct the 3D volume using the block outlines to account for jitter in the position of the cryostat head. The brain volume was then automatically segmented using seed based region growing algorithm in the Analyze software package (AnalyzeDirect, Lenexa, KS).”
Keywords
Animals
Blood-Brain Barrier/pathology/physiopathology
Cell Movement/physiology
Disease Models, Animal
Encephalitis/etiology/pathology/virology
Glial Fibrillary Acidic Protein/metabolism
HIV Infections/complications
Imaging, Three-Dimensional
Macrophages/pathology/physiology
Magnetic Resonance Imaging (MRI)
Male
Mice
Mice, SCID
Vimentin/metabolism
Author Affiliation(s)
Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha, NE 68198-5880, US. (YL, MGU, HD, RB, CBG, DKS, HEG, MDB)
Departments of Radiology, University of Nebraska Medical Center, Omaha, NE 68198-5880, US. (YL, MGU, MDB)
Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198-5880, US. (HD, RB, CBG, DKS, HEG)
Baxter Healthcare, Corporation, Round Lake, IL 60073, US. (BER)
ID# 1419
Tags: Animal Disease Models, Animals, Blood-Brain Barrier/pathology/physiopathology, Cell Movement/physiology, Encephalitis/etiology/pathology/virology, Glial Fibrillary Acidic Protein/metabolism, HIV Infections/complications, Macrophages/pathology/physiology, Magnetic Resonance Imaging (MRI), Male, Mice, SCID Mice, Three-Dimensional Imaging, Vimentin/metabolism