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Komar-Stossel C, Gross E, Dery E, Corchia N, Meir K, Fried I, Abramovitch R. TL-118 and gemcitabine drug combination display therapeutic efficacy in a MYCN amplified orthotopic neuroblastoma murine model–evaluation by MRI. PLoS One. March 2014;9(3):e90224.

Full Text – Open Access

Publication Date
March 2014

How Analyze was Used
“Tumor volume was manually assessed from the T2W images using Analyze-7.0… For each subject, an exponential growth curve was fitted to the tumor volume data points… and exponential coefficients (b-values) for each subject were isolated. The b-values, characterizing tumor growth kinetics were used as a representative value of each subject growth rate. The difference between groups was analyzed by two-sided Mann-Whitney U test with Bonferroni correction for small numbers. Survival curves were constructed using the Exact Randomization test and statistical significance was determined by the Log-Rank test (Hintze J 2007, NCSS, UT).”

“HRI maps were generated as reported previously using IDL (Interactive Data Language of ITT Visual Information Solutions, Boulder, Colorado). Tumor-ROI including the entire lesion and liver and kidney ROI’s were defined by analysis of the T2W images using the Analyze-7.0 software. Mean DS values of these ROIs were calculated by including only pixels with a statistical threshold of P<.05 (active pixels), as calculated by the one-sided Mann-Whitney U test. The percentage of active pixels was calculated for each gas challenge. All values are expressed as means 6 SD. The difference between groups was analyzed by the two-sided Mann-Whitney U test. Statistical analyses were performed with the Instat Biostatistics software (GraphPad Software Inc. San Diego, California). A P-value of <.05 was considered statistically significant.”

Keywords
Angiogenesis
Apoptosis
Blood Vessels
Cancer Treatment
Cell Proliferation
Drug Therapy
Histology
Magnetic Resonance Imaging (MRI)

Author Affiliation(s)
The Goldyne Savad Institute for Gene Therapy, Hadassah Hebrew University Medical Center, Jerusalem, Israel. (CK-S, ED, NC, RA)

MRI/MRS lab HBRC, Hadassah Hebrew University Medical Center, Jerusalem, Israel. (CK-S, ED, NC, RA)

Pediatric Surgery, Hadassah Hebrew University Medical Center, Jerusalem, Israel. (EG)

Pathology, Hadassah Hebrew University Medical Center, Jerusalem, Israel. (KM)

Pediatric Hemato-Oncology, Hadassah Hebrew University Medical Center, Jerusalem, Israel. (IF)

ID# 134

Tags: Angiogenesis, Apoptosis, Blood Vessels, Cancer Treatment, Cell Proliferation, Drug Therapy, Histology, Magnetic Resonance Imaging (MRI)