AnalyzeDirect Hongru Gao et al. Three-Dimensional Visualization of Electroacupuncture Activates Brown Adipose Tissue via Sympathetic Innervation in PCOS Rats ResearchSquare.
Publication Date
March 3, 2022
How Analyze was Used
“Eight weeks after modeling and at the end of the EA treatment, micro CT (Quantum; PerkinElmer, Hopkinton, MA, USA) was carried out to acquire images of BAT and WAT. The rats were anesthetized continuously by isoflurane (2% in a 1:1 mixture of oxygen and air, RWD Life Science Co., Shenzhen, China) with a mask in the CT machine. The X-ray parameters were 114 µA and 70 kVp. The scanning process was managed by a computer, and the image field was 72 mm × 40 mm with 144 voxels. Analyze 12.0 software (Analyze Direct, KS, USA) was used to acquire the CT images and for image segmentation, and the target area module and volume editing tools in the software were used to quantify the BAT volume.”
Keywords
Polycystic ovary syndrome, Electroacupuncture, Brown adipose tissue, Sympathetic nerve,
Mitochondrial brown fat uncoupling protein 1
Author Affiliation(s)
Fudan University, Shanghai, China
