AnalyzeDirect Eun Jung Yim et al. Protection against Osteoporosis by Fermented Mulberry Vinegar Supplementation via Inhibiting Osteoclastic Activity in Ovariectomized Rats and Osteoclastic Cells Fermentation.
Publication Date
May 5, 2022
How Analyze was Used
“The femur was fixed with 4% paraformaldehyde, and its bone status was measured. Micro-CT imaging was performed using a Quantum GX Micro-CT imaging system (PerkinElmer, Hopkinton, MA, USA), located at the Korea Basic Science Institute (Gwangju, Korea). The X-ray source was set to 90 kV and 88 mA levels with a field of view of 10 mm (voxel size, 20 µm; scanning time, 4 min). The 3D images were visualized with a 3D Viewer consisting of software within the Quantum GX, and its resolution was set at 4.5 µm. The
structural parameters for trabecular bone were analyzed following scanning using the Analyze 12.0 software (Analyze Direct, Overland Park, KS, USA). The BMD for the femur was estimated using a hydroxyapatite phantom (QRM-MicroCT-HA, QRM GmbH, Möhrendorf, Germany) with scanned the femur using the same parameters. We calculated BMD, total volume (TV), bone volume (BV), bone surface (BS), bone surface density (BS/TV), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp), and trabecular number (Tb.N) of the femur.”
Keywords
Osteoporosis, Osteoclastic activity, Mulberry vinegar, RANKL, TRAP, Osteoprotegerin
Author Affiliation(s)
Hoseo University, Asan, Korea
