AnalyzeDirect Vardit Kram et al. OPG-Fc treatment partially rescues low bone mass phenotype in mature Bgn/Fmod deficient mice but is deleterious to the young mouse skeleton Journal of Structural Biology.
Publication Date
September 17, 2020
How Analyze was Used
“Skull scans were exported to Analyze 14.0 software (AnalyzeDirect, KS, USA) for further analysis. All skulls were oriented so that the transverse plane was parallel to x-axis, whereas the coronal and sagittal planes were perpendicular. Since the skull is composed of complex bone structures of varying densities, we set two different thresholds during µCT segmentation. The first threshold was set to define bone of normal density (ND) at 0.6g HA/cm3. A second threshold defined finer bone structures of lower density (LD bone) than the rest of the skull at 0.3 to 0.59g HA/cm3 (e.g., the squamous part of the temporal bone). In order to calculate the total volume for each skull, an elliptical shape was fabricated to encompass the skull. Mandibles and teeth were excluded from the final region of interest (ROI). The Bone Microarchitecture add-on in Analyze 14.0 was used to analyze the segmented skulls. In order to segment the occipital bone, the boundaries were first demarcated on the 3D rendering along the suture lines of occipital bone using the freehand tool in Analyze. Following the demarcation of outline, the body of occipital bone was segmented manually in the sagittal sections using the freehand tool. Thresholding similar to the whole skull (ND Bone and LD Bone) was performed to look at changes in parameters specific to this bone. Five mice per group were analyzed.”
Keywords
Proteoglycans, Biglycan, Fibromodulin, Osteoporosis, Osteoclast, Ectopic ossification, OPG-Fc, Low Bone Mass
Author Affiliation(s)
National Institutes of Health, Bethesda, MD 20892
