AnalyzeDirect

Klohs J, Steinbrink J, Bourayou R, Mueller S, Cordell R, Licha K, Schirner M, Dirnagl U, Lindauer U, Wunder A. Near-infrared fluorescence imaging with fluorescently labeled albumin: a novel method for non-invasive optical imaging of blood-brain barrier impairment after focal cerebral ischemia in mice. J Neurosci Methods. May 2009;180(1):126-132.

Publication Date
May 2009

How Analyze was Used
“On post-contrast images, ROIs were defined in the ipsilateral, ischemic hemisphere, its mirror image over the contralateral, non-ischemic hemisphere using Analyze software (AnalyzeDirect, Inc., Lenexa, USA).”

Keywords
Albumins/chemistry/diagnostic use/pharmacokinetics
Animals
Blood-Brain Barrier/metabolism/pathology/physiopathology
Brain Edema/etiology/pathology/physiopathology
Brain Ischemia/complications/pathology/physiopathology
Evans Blue/diagnostic use/pharmacokinetics
Fluorescent Dyes/chemistry/diagnostic use/pharmacokinetics
Gadolinium DTPA/diagnostic use/pharmacokinetics
Infarction, Middle Cerebral Artery/etiology/pathology/physiopathology
Magnetic Resonance Imaging (MRI)/methods
Male
Mice
Mice, Inbred C57BL
Microscopy, Fluorescence/methods
Serum Albumin, Bovine/chemistry/diagnostic use/pharmacokinetics
Spectroscopy, Near-Infrared/methods

Author Affiliation(s)
Department of Experimental Neurology, Charité – University Medicine Berlin, Germany. (JK, RC, UD, UL, AW)

Center for Stroke Research Berlin (CSB), Charité – University Medicine Berlin, Germany. (JK, JS, SM, UD, AW)

Berlin NeuroImaging Center, Charité – University Medicine Berlin, Germany. (JS, RB, SM)

NEUROCURE Research Center, Berlin, Germany. (UD)

mivenion GmbH, Berlin, Germany. (KL, MS)

ID# 1302

Tags: Albumins/chemistry/diagnostic use/pharmacokinetics, Animals, Blood-Brain Barrier/metabolism/pathology/physiopathology, Bovine Serum Albumin/chemistry/diagnostic use/pharmacokinetics, Brain Edema/etiology/pathology/physiopathology, Brain Ischemia/complications/pathology/physiopathology, Evans Blue/diagnostic use/pharmacokinetics, Fluorescence Microscopy/methods, Fluorescent Dyes/chemistry/diagnostic use/pharmacokinetics, Gadolinium DTPA/diagnostic use/pharmacokinetics, Inbred C57BL Mice, Magnetic Resonance Imaging (MRI)/methods, Male, Mice, Middle Cerebral Artery Infarction/etiology/pathology/physiopathology, Near-Infrared Spectroscopy/methods