Analyze Publications Database

Monitoring bacterial burden, inflammation and bone damage longitudinally using optical and muCT imaging in an orthopaedic implant infection in mice

Niska JA, Meganck JA, Pribaz JR, Shahbazian JH, Lim E, Zhang N, Rice BW, Akin A, Ramos RI, Bernthal NM, Francis KP, Miller LS. Monitoring bacterial burden, inflammation and bone damage longitudinally using optical and muCT imaging in an orthopaedic implant infection in mice. PLoS One. October 2012;7(10):e47397.

Full Text – Open Access

Publication Date
October 2012

How Analyze was Used
“A 3D region of interest was created by propagating between these contours and volume of this ROI was measured (Analyze 10.0, AnalyzeDirect Inc., Overland Park, KS). To visualize these results, the reoriented image was loaded and a 3D rendering was generated from the anterolateral perspective using a fixed threshold range across all of the time points.”

Keywords
Animals
Bacterial Load
Bone and Bones/pathology/radiography
Fluorescence
Implants, Experimental/adverse effects
Inflammation/complications/pathology/radiography
Knee Joint/microbiology/pathology/radiography/surgery
Male
Mice
Neutrophil Infiltration
Optical Imaging
Orthopedics
Prosthesis-Related Infections/complications/microbiology/pathology/radiography
Staphylococcus aureus/growth & development
X-Ray Microtomography

Author Affiliation(s)
Orthopaedic Hospital Research Center, Orthopaedic Hospital Department of Orthopaedic Surgery, David Geffen School of Medicine at University of California Los Angeles (UCLA), Los Angeles, CA, US. (JAN, JRP, NMB)

Caliper – a PerkinElmer Company, Alameda, CA, US. (JAM, EL NZ, BWR, AA, KPF)

Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, MD, US. (JHS, RIR, LSM)

Novartis Institutes for BioMedical Research, Inc., Emeryville, CA, US. (AA)

ID# 488

Tags: , , , , , , , , , , , , , ,