AnalyzeDirect E. Tian et al. Loss of the glycosyltransferase Galnt11 affects vitamin D homeostasis and bone composition Journal of Biological Chemistry.
Publication Date
March 12, 2024
How Analyze was Used
“Analyze (version 14.0; AnalyzeDirect, Overland Park, KS, USA) was used to reconstruct and analyze images. Reconstructed images were calibrated to a linear standard curve using five hydroxyapatite (HA) phantoms with known densities (mg/cm3). Both trabecular and cortical volumes of interest (VOI) were defined for each femur according to standard algorithms. In brief, the growth plate cross-section served as an anatomical reference from which the long axis of the volume of interest (VOI) was defined. The trabecular VOI was defined to begin at 67% of the proximal-to-distal length of the femur and extend distally to 0.6 mm proximal from the growth plate. The cortical VOI was defined to start at 56% of the proximal-to-distal length of the femur and extend 1 mm distally toward the growth plate. The minimum threshold for trabecular bone was defined as 300 (mg HA/cm3) and the minimum threshold for cortical bone was defined as 550 (mg HA/cm3). Morphometrics and densities were then calculated for trabecular and cortical regions.”
Keywords
Galnt11, O-glycosylation, Megalin, Vitamin D binding protein, Bone remodeling, Cortical bone, Trabecular bone, Vitamin D, Calcium, Parathyroid hormone, Kidney
Author Affiliation(s)
National Institutes of Health, Bethesda, Maryland USA
