AnalyzeDirect Yuria Jang et al. Inhibition of RANKL-Induced Osteoclastogenesis by Novel Mutant RANKL
International Journal of Molecular Sciences.
Publication Date
January 6, 2021
How Analyze was Used
“Micro-CT scanning for the distal femur was distally initiated at the level of the growth plate using a Quantum GX micro-CT imaging system (PerkinElmer, Hopkinton, MA, USA) located at the Korea Basic Science Institute in Gwangju, Korea. The scanning X-ray source was set to 90 kV and 88 mA with a field of view of 10 mm (voxel size, 20 µm; scanning time, 4 min). The 3D imaging was performed via 3D Viewer, an existing software within the Quantum GX system. The resolution was set at 4.5 µm and images were obtained. Following scanning, the structural parameters for the trabecular bone were analyzed using the Analyze 12.0 software (AnalyzeDirect, Overland Park, KS, USA). The mineral density of the femur was estimated using a hydroxyapatite (HA) phantom (QRM-MicroCT-HA, Quality Assurance in Radiology and Medicine GmbH, Möhrendorf, Germany), which was scanned using the same parameters. The bone mineral density (BMD), % of bone volume (bone volume/tissue volume, %), trabecular number (Tb. N.), trabecular separation (Tb. Sp.), and trabecular thickness of the femurs were calculated using the ROI tool. Parameters values are shown as mean ± standard deviation (SD).”
Keywords
Leucine-rich repeat-containing G-protein-coupled receptor 4, Receptor activator of nuclear
factor kappa-B ligand, Osteoclast, Osteoporosis
Author Affiliation(s)
Chosun University Hospital, Dong-Gu, Gwangju, Korea
