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Development of a miniaturized 96-Transwell air–liquid interface human small airway epithelial model

Teresa Bluhmki et al. Development of a miniaturized 96-Transwell air–liquid interface human small airway epithelial model. Scientific Reports.

Publication Date
August 3, 2020

How Analyze was Used
“Visualization of the image stacks was performed using the Analyze software (AnalyzeDirect, Overland Park, KS). A grey value time course was calculated for each pixel over 512 frames per region. The mean ciliary beating frequency of a region was determined by calculating the average frequency for the cyclic changes of the grey scale. The area covered by motile cilia was determined by quantifying the percentage of pixels with a measurable beating frequency within a region.”

Keywords
Epithelium, Biochemistry, Biomarkers, Cell biology, Drug discovery

Author Affiliation(s)
Boehringer Ingelheim Pharma GmbH & Co. KG, Biberach an der Riß, Germany

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