AnalyzeDirect Sieck GC, Han YS, Pabelick CM, Prakash YS. Temporal aspects of excitation-contraction coupling in airway smooth muscle. J Appl Physiol (1985). November 2001;91(5):2266-2274.
Full Text – Open Access
Publication Date
November 2001
How Analyze was Used
“The images were transferred to a processing software (ANALYZE …), in which the delay between elevation in [Ca2+]i (>10 gray level increase in pixel intensity from baseline) and the initiation of contraction was measured by detecting the edges of a myocyte and automatically tracking for movement of the edge.”
Keywords
Acetylcholine/pharmacology
Animals
Calcium/metabolism
In Vitro Techniques
Muscle Contraction/physiology
Muscle Relaxation/physiology
Muscle, Smooth/cytology/metabolism/physiology
Perfusion
Photolysis
Solutions
Swine
Time Factors
Trachea/cytology/metabolism/physiology
Author Affiliation(s)
Department of Anesthesiology, Mayo Clinic and Foundation, Rochester, MN 55905, US. (GCS, YSH, CMP, YSP)
Department of Physiology and Biophysics, Mayo Clinic and Foundation, Rochester, MN 55905, US. (GCS)
ID# 2719
Tags: Acetylcholine/pharmacology, Animals, Calcium/metabolism, In Vitro Techniques, Muscle Contraction/physiology, Muscle Relaxation/physiology, Perfusion, Photolysis, Smooth Muscle/cytology/metabolism/physiology, Solutions, Swine, Time Factors, Trachea/cytology/metabolism/physiology