Alison Domingues et al. Targeting endothelial thioredoxin-interacting protein (TXNIP) protects from metabolic disorder-related impairment of vascular function and post-ischemic revascularisation. Angiogenesis.
January 3, 2020
How Analyze was Used
“Following scanning, image-processing steps were undertaken. After acquiring 360° projections, 3D reconstructions were obtained using RigakuSW (Caliper) software (voxels matrix of 512 × 512 × 512 with 20 µm resolution) and analysed with Analyze 11.0. Images were thresholded to select the vessels and separated from the other tissues (soft tissue and bone tissue). After segmentations, vascular density and the distribution of the vessels according to their calibre were calculated. For vascular density (%), analyses were performed with Region of Interest (ROI) in Analyze 11.0. Vascular density was then calculated reporting vessel segmentation in soft tissues (fat, muscle…). For calculation of the number of small vessels, in the same ROI as for vascular density, the events were counted with Object Counter module in Analyze 11.0 for each projection and distributed, according to their calibre. To overcome potential under-filling of contrast product, analysis was achieved by normalisation of the ischemic leg to the non-ischemic control leg.”
TXNIP, Metabolic disorders, Endothelial dysfunction, Oxidative stress, Arterial damage, Post-ischemic revascularisation
Université de Paris, Paris, France