Tag Archives: Transfection

Imaging and analysis of three-dimensional cell culture models

Graf BW, Boppart SA. Imaging and analysis of three-dimensional cell culture models. Methods Mol Biol. January 2010;591:211-227. Full Text – Open Access Publication Date January 2010 How Analyze was Used “Using the Analyze software, en face planes are computationally extracted from the 3D data to observe the structure (see Fig. 13.6a) and functional (see Fig….

Agonist-dependent internalization of D2 receptors: imaging quantification by confocal microscopy

Goggi JL, Sardini A, Egerton A, Strange PG, Grasby PM. Agonist-dependent internalization of D2 receptors: imaging quantification by confocal microscopy. Synapse. April 2007;61(4):231-241. Publication Date April 2007 How Analyze was Used “Images were then analyzed using Analyze 6.0 software (AnalyzeDirect, Lenexa, KS). Images were smoothed by using a 2 x 2 x 2 smoothing kernel…

Imaging cellular responses to mechanical stimuli within three-dimensional tissue constructs

Tan W, Vinegoni C, Norman JJ, Desai TA, Boppart SA. Imaging cellular responses to mechanical stimuli within three-dimensional tissue constructs. Microsc Res Tech. April 2007;70(4):361-371. Publication Date April 2007 How Analyze was Used “Three-dimensional visualization of the engineered tissue constructs was achieved by volume rendering the image stacks of OCM and MPM data using 3D…

Second-generation triple reporter for bioluminescence, micro-positron emission tomography, and fluorescence imaging

Kesarwala AH, Prior JL, Sun J, Harpstrite SE, Sharma V, Piwnica-Worms D. Second-generation triple reporter for bioluminescence, micro-positron emission tomography, and fluorescence imaging. Mol Imaging. October 2006;5(4):465-474. Full Text – Open Access Publication Date October 2006 How Analyze was Used “Regions of interest were selected manually and analyzed for radioactivity content (μCi/g tissue/μCi injected dose/g…

A functional aquaporin co-localizes with the vacuolar proton pyrophosphatase to acidocalcisomes and the contractile vacuole complex of Trypanosoma cruzi

Montalvetti A, Rohloff P, Docampo R. A functional aquaporin co-localizes with the vacuolar proton pyrophosphatase to acidocalcisomes and the contractile vacuole complex of Trypanosoma cruzi. J Biol Chem. September 2004;279(37):38673-38682. Full Text – Open Access Publication Date September 2004 How Analyze was Used “The surface area of the sequential images was calculated using AnalyzeAVW version…

Rapid internalization and recycling of the human neuropeptide Y Y1 receptor

Gicquiaux H, Lecat S, Gaire M, Dieterlen A, Mély Y, Takeda K, Bucher B, Galzi JL. Rapid internalization and recycling of the human neuropeptide Y Y1 receptor. J Biol Chem. February 2002;277(8):6645-6655. Full Text – Open Access Publication Date February 2002 How Analyze was Used “Co-localization analysis was done using AnalyzeAVW 3.0 software installed on…