AnalyzeDirect Eriksson SH, Free SL, Thom M, Symms MR, Martinian L, Duncan JS, Sisodiya SM. Quantitative grey matter histological measures do not correlate with grey matter probability values from in vivo MRI in the temporal lobe. J Neurosci Methods. June 2009;181(1):111-118.
Full Text – Open Access
Publication Date
June 2009
How Analyze was Used
“The ROI was also defined on the MRI T1-volume data used for the correlation with histopathology (reformatted to oblique coronal images with a voxel size of 0.94 mm3) using Analyze AVW 5.0 … The ROIs were outlined on each patient’s T1-weighted scan and was not the same for each patient since the anatomy of the temporal lobes differed between the patients. The ROIs (cortical crown of the middle temporal gyrus) were extracted in subject space by manual drawing guided by visual inspection of the previously determined MR:pathology correlation. This step used the same criteria to characterise the limits of the gyral crown as were used in the quantitative neuropathology. The data from the initial MR:pathology correlations were used to determine which MR slices within the middle temporal gyrus corresponded to the ROI in each subject (Fig. 1). The manual outlining of the ROI was performed on the T1-weighted images rather than the segmented images to make use of the previous MR:pathology co-registration and ensure we were analysing the same areas on both MRI and histopathology in each patient.”
Keywords
Adult
Brain Mapping
Electroencephalography
Epilepsy, Temporal Lobe/pathology/surgery
Female
Glial Fibrillary Acidic Protein/metabolism
Humans
Image Processing, Computer-Assisted/methods
Magnetic Resonance Imaging (MRI)/methods
Male
Middle Aged
Neuroglia/metabolism/pathology
Neurons/metabolism/pathology
Phosphopyruvate Hydratase/metabolism
Probability
Statistics as Topic
Temporal Lobe/pathology
Young Adult
Author Affiliation(s)
Department of Clinical and Experimental Epilepsy, UCL Institute of Neurology, London WC1N3BG, UK.
ID# 1396
Tags: Adult, Brain Mapping, Computer-Assisted Image Processing/methods, Electroencephalography, Female, Glial Fibrillary Acidic Protein/metabolism, Humans, Magnetic Resonance Imaging (MRI)/methods, Male, Middle Aged, Neuroglia/metabolism/pathology, Neurons/metabolism/pathology, Phosphopyruvate Hydratase/metabolism, Probability, Statistics as Topic, Temporal Lobe Epilepsy/pathology/surgery, Temporal Lobe/pathology, Young Adult