Tanner Stokes et al. Methodological considerations for and validation of the ultrasonographic determination of human skeletal muscle hypertrophy and atrophy Physiological Reports.
January 6, 2021
How Analyze was Used
“Peak quadriceps CSA (i.e., the cross‐sectional MRI image slice for which the quadriceps CSA was maximal) and quadriceps volume were quantified using semi‐automatic software, Analyze 14.0 (AnalyzeDirect, Overland Park, NS) as previously described (Holloway et al., 2019). First, the distal 20% of thigh images and the proximal 30% of MRI images from each leg were excluded from analysis due to an increased difficulty to differentiate muscle groups at these extremes. Total quadriceps CSA was subsequently traced manually on every other image slice. The tracings were propagated to untraced images and used to train an algorithm to differentiate muscle from adipose and connective tissue. These images were in turn randomly checked for accuracy. Muscle volume (cm3) was calculated as the sum of CSA values from all of the analyzed image slices multiplied by the slice thickness.”
Exercise, Human, Imaging, Immobilization
McMaster University, Hamilton, Ontario, Canada