AnalyzeDirect Flavia Amadeu de Oliveira et al. Gene therapy using recombinant AAV type 8 vector encoding TNAP-D10 improves the skeletal phenotypes in murine models of osteomalacia Journal of Bone and Mineral Research Plus.
Publication Date
December 1, 2022
How Analyze was Used
“Femurs were scanned using a SkyScan 1172 scanner (Bruker Micro-CT, Kontich, Belgium) at 55 kV, 181 μA, 0.5 mm Al filter, 280 ms integration time, and 10 μm voxel dimension. Micro-CT images were reconstructed in NRecon software and were calibrated to three known densities of hydroxyapatite. Analysis was done using AnalyzePro (version 1.0; AnalyzeDirect, Overland Park, KS). Trabecular and cortical bone of femurs were segmented at 400 and 550 mg HA/cm3, respectively. Micro-CT analysis of femurs was performed as previously described. Briefly, to quantify the trabecular bone parameters, such as bone volume (Tb. BV), total volume (Tb.TV), bone volume fraction (Tb.BV/TV), trabecular number (Tb.N), thickness (Tb.Th), spacing (Tb.Sp), connective density (Tb.Conn.D), and mineral density (Tb.BMD), a total of 0.5 mm (50 slices) proximal to the distal femur growth plate was traced. For the cortical bone, 50 slices of the mid-femur of each bone were used to quantify cortical bone volume fraction (Ct.BV/TV), cortical thickness (Ct.Th), marrow area (Ma. Ar), and mineral density (Ct.BMD).”
Keywords
Gene therapy, Recombinant AAV type 8 vector encoding, TNAPD10, Skeletal phenotypes, Murine models, Osteomalacia
Author Affiliation(s)
Sanford Burnham Prebys Medical Discovery Institute, La Jolla, California, USA
