AnalyzeDirect Laia Morató et al. eNAMPT actions through nucleus accumbens NAD+/SIRT1 link increased adiposity with sociability deficits programmed by peripuberty stress Science Advances.
Publication Date
March 2, 2022
How Analyze was Used
“Abdominal fat volume was determined by x-ray microCT (Quantum Fx Micro CT, PerkinElmer), a noninvasive, quantitative technique to determine the spatial distribution of specific fat compartments. Scan conditions were set as follows: voltage = 90 kV, current = 160 μA, live = 80 μA, field of view = 24 mm, scan mode = fine (2 min), and voxel size = 50 μm. Anesthesia was maintained by mask inhalation of isoflurane vaporized at concentrations of up to 3% in the induction phase and at 1.5% during acute imaging procedure. Three-dimensional images were reconstructed and quantified using the visualization and imaging software Analyze 12.0 (AnalyzeDirect Inc., KS, USA) by an experienced researcher blinded to the experimental group. The abdominal muscular wall was used to separate visceral adipose tissue from subcutaneous adipose tissue. Fat depot volumes were normalized to the body weight of the mice.”
Keywords
eNAMPT, Nucleus accumbens, NAD+/SIRT1, Adiposity, Sociability, Peripuberty stress
Author Affiliation(s)
Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
