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Thormann U, El Khawassna T, Ray S, Duerselen L, Kampschulte M, Lips K, von Dewitz H, Heinemann S, Heiss C, Szalay G, Langheinrich AC, Ignatius A, Schnettler R, Alt V. Differences of bone healing in metaphyseal defect fractures between osteoporotic and physiological bone in rats. Injury. March 2014;45(3):487-493.

Full Text – Open Access

Publication Date
March 2014

How Analyze was Used
“To obtain the vascular volume formation (VVF) by means of μCT (n = 6/group), the abdominal aorta was cannulated and infused with heparinized saline (10 ml of 0.9% sodium chloride with 1000 IU Heparin) immediately after euthanasia until the venous effluent was free of blood. A lead-containing radiopaque polymer (Microfil® MV-122, Flow Tech, Carver, MA, USA) was used to carry out the perfusion. It was administered into the aorta and following polymerization of the compound, the femora with the indwelling mini-plate were removed and immediately fixed in 4% phosphate buffered paraformaldehyde.

“New ossified tissue formation was assessed within the fracture gap and on the lateral periosteal site. Longitudinal cross-section parallel to the specimen’s long axis was reconstructed using en-bloc and measurement of total ossified tissue was performed. Axial cross-sections within the fracture zone were identified and the cross-sectional area (mm²) of total ossified tissue was measured using the ANALYZE software (ANALYZE 10.0).”

Keywords
Bone Healing
Fracture
Metaphysis
Osteoporosis
Osteotomy

Author Affiliation(s)
Department of Trauma Surgery, University Hospital of Giessen-Marburg, Giessen, Germany. (UT, CH, GS, RS, VA)

Laboratory of Experimental Trauma Surgery, Justus-Liebig University, Giessen, Germany. (UT, TEK, SR, KL, CH, RS, VA)

Institute of Orthopedic Research and Biomechanics, Centre of Musculoskeletal Research, University of Ulm, Ulm, Germany. (LD, AI)

Department of Radiology, University Hospital of Giessen-Marburg, Giessen, Germany. (MK, HD)

Institute of Materials Science, Technische Universität Dresden, Dresden, Germany. (SH)

Department of Diagnostic and Interventional Radiology, BG Trauma Hospital Frankfurt/Main, Germany. (ACL)

ID# 158

Tags: Bone Healing, Fracture, Metaphysis, Osteoporosis, Osteotomy