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Moritz Gröschel¹, Romy Götze¹, Susanne Müller², Arne Ernst¹, Dietmar Basta¹. Central Nervous Activity upon Systemic Salicylate Application in Animals with Kanamycin-Induced Hearing Loss – A Manganese-Enhanced MRI (MEMRI) Study. PLoS One. April 14th, 2016.

Full Text – Open Access

Publication Date
April 2016

How Analyze was Used
“Signal intensity analysis of defined auditory brain regions was carried out with the program Analyze 5.0 (AnalyzeDirect, Inc.; Lenexa USA). Signal intensities were quantified bilaterally in the dorsal (DCN) and ventral (VCN) cochlear nucleus, the superior olivary complex (SOC), the inferior colliculus (IC), the medial geniculate body (MGB), the auditory cortex (AC), the preoptic area of the anterior hypothalamus (PO/AH), the Amygdala (Amyg). The masseter muscle was used as an objective intensity reference, since it is located close to the related neural tissue but is not affected by changes in brain activity. PO/AH and Amyg served as non-auditory structures investigated in the present study. Regions of interest were marked in accordance with “the mouse brain atlas in stereotaxic coordinates” [73] (Fig 1). The investigated brain structures were manually outlined within each relevant brain slice and a voxel-based analysis was carried out for each structure under observation. Due to variations in each animal’s position within the MRI scanner, the dimensions of each brain structure (the tissue volume) in the scan could vary across subjects. Therefore, the number of voxels included in each structure differs slightly. Signal intensities for all analysed structures were normalized in relation to the intensity of the muscle at the same side. The relative MRI signal (given in normalized relative units) was calculated for each animal by using the mean of the measured signal strength of every slice for each analysed structure, normalized in relation to the intensity for the muscle at that side. The results of all animals were compared between the salicylate group and the hearing-impaired control group for each investigated brain area.”

Keywords
Salicylates
Magnetic resonance imaging
Deafness
Tinnitus
Amygdala
Auditory pathway
Auditory system
Manganese

Author Affiliation(s)
¹Department of Otolaryngology, Unfallkrankenhaus Berlin, Charité Medical School, Berlin, Germany
²Neuroscience Research Center (NWFZ), Charité Medical School, Berlin, Germany

Tags: Amygdala, Auditory Pathways, Deafness/radiography/surgery, Magnetic Resonance Imaging, Manganese, Tinnitus/physiopathology/therapy