AnalyzeDirect David J. Lillyman et al. Axial hypersensitivity is associated with aberrant nerve
sprouting in a novel model of disc degeneration in female
Sprague Dawley rats JOR Spine.
Publication Date
July 14, 2022
How Analyze was Used
“The L5-L6 disc volume was quantified using the Quantum GX2 μCT Imaging System and Analyze(Pro) (AnalyzeDirect). In short, the rat lumbar spine was radiographed by placing anesthetized animals in the supine position and scanning for 2-min with 90 kV power, 88 μA tube current, 72 mm FOV, 144 um voxel size, and a Cu 0.06 + Al 0.05 x-ray filter. VOX files were then removed from the μCT computer and analyzed in Analyze(Pro). To begin processing, raw VOX files from the μCT scans were processed using a high pass threshold to remove all non-bony signal. After the scans were reduced to only bony tissue using the software filter, the intervertebral disc space was colored in every coronal plane where the adjacent vertebral bodies were present using a manual draw tool. The slices of colored intervertebral disc space between the L5 and L6 vertebral bodies were then concatenated, smoothed using a built-in function and saved as an object map. The volume of the object map of these concatenated colored planes was quantified using a built-in software analysis.”
Keywords
Axial hypersensitivity, Disc degeneration, Disc-associated pain, Discogenic pain, Grip strength,
Low back pain, Nerve sprouting, Open arena, Pressure algometry
Author Affiliation(s)
University of Nebraska, Lincoln, Nebraska, USA
