AnalyzeDirect Ramkumar Thiyagarajan et al. Age-related increase of CD38 directs osteoclastogenic potential of monocytic myeloid-derived suppressor cells through mitochondrial dysfunction in male mice Aging Cell.
Publication Date
August 23, 2024
How Analyze was Used
“Calibrated three-dimensional images were generated, reconstructed, and analyzed using AnalyzePro software (AnalyzeDirect, Inc., Overland Park, KS). The parameters of trabecular bone were measured within a specific region of interest located 80 slices (960 μm) from the opening of the epiphyseal growth plate. To select a consistent metaphysis, 80 slices were chosen, starting from 120 slices below to the growth plate to calculate the cortical bone parameters. All images were analyzed individually, and the ROI was applied. The following trabecular and cortical parameters were calculated using AnalyzePro software: bone volume fraction (BV/TV, %), trabecular thickness (Tb.Th, mm), trabecular separation (Tb.Sp, mm), trabecular number (Tb.N, 1/mm), cortical bone area (Ct. Ar), the total cross-sectional area inside the periosteal envelope (Tt.Ar), cortical area fraction (Ct.Ar/Tt.Ar), and average cortical thickness (Ct.Th). Bone histological assessments were conducted based on our recent publication (Zhang et al., 2024).”
Keywords
Aging, Bone resorption, Metabolism, Myeloid-derived suppressor cells, Osteoclasts
Author Affiliation(s)
University of Kansas School of Medicine, Kansas City, Kansas, USA
