McLaren JW, Patel SV, Nau CB, Bourne WM. Automated assessment of keratocyte density in clinical confocal microscopy of the corneal stroma. J Microsc. January 2008;229(Pt 1):21-31.
Full Text – Open Access
Publication Date
January 2008
How Analyze was Used
“A program was developed to assess cell density in confocal images by using a scripted language, Tcl/tk (“tool command language/toolkit,” …) and an image processing program, Analy[z]e AVW… The program worked in two steps; first, it identified bright objects in the field, and second, it selected those objects that most likely corresponded to cells based on the image properties of each object.”
Keywords
Corneal Stroma/cytology
Fibroblasts/cytology
Humans
Keratomileusis, Laser In Situ
Microscopy, Confocal/methods
Myopia/surgery
Author Affiliation(s)
Department of Ophthalmology, Mayo Clinic College of Medicine, Rochester, MN 55905, US.
ID# 1605
Tags: Confocal Microscopy/methods, Corneal Stroma/cytology, Fibroblasts/cytology, Humans, Laser In Situ Keratomileusis, Myopia/surgery