AnalyzeDirect Bernard CE, Gibbons SJ, Gomez-Pinilla PJ, Lurken MS, Schmalz PF, Roeder JL, Linden D, Cima RR, Dozois EJ, Larson DW, Camilleri M, Zinsmeister AR, Pozo MJ, Hicks GA, Farrugia G. Effect of age on the enteric nervous system of the human colon. Neurogastroenterol Motil. July 2009;21(7):746-e746.
Full Text – Open Access
Publication Date
July 2009
How Analyze was Used
“Normal descending and sigmoid colonic tissue was obtained as surgical surplus tissue as a result of hemicolectomies carried out for non-obstructing colon cancer.”
“Seven slides per patient were labelled for Hu, four for ChAT and three for PGP 9.5 and for nNOS. There were two sections per slide and all tissue on each section was examined. Sections that were not properly oriented were discarded. Number of ganglia and number of neurons in both the submucosal and myenteric plexuses were counted and expressed as number of neurons/ganglion and number of neurons/mm length. A ganglion was defined as a structure containing more than one neuron. This method of quantification avoided problems where the ganglia in the sections were not uniformly distributed or where the section was not straight. This is particularly true for the submucosal plexus which is diffuse and has large spaces between ganglia. We applied a trace tool in the software program Analyze™ … to draw a line that followed the contours of the plexuses (Fig. 1A). The line delineated the length of the section. Within that length, all neurons and ganglia in the myenteric and submucosal areas were counted (Fig. 1B).”
Keywords
Adult
Aged
Aged, 80 and over
Aging/metabolism/pathology
Cell Count
Choline O-Acetyltransferase/metabolism
Colon/cytology/innervation/metabolism
Enteric Nervous System/cytology/metabolism
Female
Hu Paraneoplastic Encephalomyelitis Antigens
Humans
Male
Middle Aged
Neurons/cytology/metabolism
Author Affiliation(s)
Enteric NeuroScience Program, Division of Gastroenterology and Hepatology, Mayo Clinic College of Medicine, Rochester, MN, US. (CEB, SJG, PJG-p, MSL, PFS, JLR, DL, MC, ARZ, GF)
Department of Physiology, Nursing School, University of Extremadura, Caceres, Spain. (PJG-p, MJP)
Department of Surgery, Mayo Clinic College of Medicine, Rochester, MN, US. (RRC, EJD, DWL)
Division of Biostatistics, Mayo Clinic College of Medicine, Rochester, MN, US. (ARZ)
Red Tematica de Investigación Cooperativa en Envejecimiento y Fragilidad, (RETICEF), Instituto de Salud Carlos III, Barcelona, Spain. (MJP)
Novartis Pharmaceuticals Corporation, East Hanover, NJ, US. (GAH)
ID# 1314
Tags: Aged, Aged_80 and over, Aging/metabolism/pathology, Cell Count, Choline O-Acetyltransferase/metabolism, Colon/cytology/innervation/metabolism, Enteric Nervous System/cytology/metabolism, Female, Hu Paraneoplastic Encephalomyelitis Antigens, Humans, Male, Middle Aged, Neurons/cytology/metabolism