AnalyzeDirect Jessica D. Hathaway-Schrader et al. Commensal oral microbiota induces osteoimmunomodulatory effects separate from systemic microbiome in mice JCI Insight.
Publication Date
January 25, 2022
How Analyze was Used
“Calibrated three-dimensional images were reconstructed. L5 vertebral bodies and tibiae were evaluated using Analyze 12.0 Bone Microarchitecture Analysis software (AnalyzeDirect). For vertebral trabecular analysis, axial CT slices were analyzed beginning 150μm coronal to the caudal epiphyseal growth plate and extending 500μm coronally; threshold=1750 Hounsfield Units (HU). For tibia cortical analysis, transverse CT slices were analyzed in a 1000μm segment of the tibia mid-diaphysis at a threshold=2500 HU. Micro-CT analyses for maxillae and mandibles were performed using AnalyzePro Segment and Measure Evaluation Program (AnalyzeDirect). Trabecular bone parameters were evaluated in the maxillary first molar trifurcation by creating a volume of interest (VOI) defined by linearly morphing two consecutive cylinder ROIs. Initial ROI began 100μm apical to the fornix/roof of the furcation and the second ROI was 200μm apical to the initial ROI. Cortical thickness was evaluated in a 200μm mesial-distal ROI in the lingual and buccal cortical plate of the mandibular first molar bifurcation. ROI was centered at the midpoint between the mesial and distal roots in the sagittal plane; measurements were then performed from 10µm to 100µm in 10µm increments distal and mesial to the midpoint. Maxilla and mandible threshold=270 HU. Data are reported in accordance with standardized nomenclature.”
Keywords
Bone Biology, Bone marrow, MHC class 2, Microbiology, Osteoclast/osteoblast biology
Author Affiliation(s)
Medical University of South Carolina, Charleston, USA
